Raman based detection of Staphylococcus aureus utilizing single domain antibody coated nanoparticle labels and magnetic trapping

Download
  1. Get@NRC: Raman based detection of Staphylococcus aureus utilizing single domain antibody coated nanoparticle labels and magnetic trapping (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1039/c3ay40652k
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical Methods
ISSN1759-9660
Volume5
Issue16
Pages41524158; # of pages: 7
Subject4-mercaptobenzoic acids; Coated nanoparticles; Detection and quantifications; Gold Nanoparticles; Pathogenic bacterium; Single-domain antibodies; Staphylococcus aureus; Surface enhanced Raman spectroscopy; Antibodies; Bacteria; Chemical detection; Gold; Iron oxides; Metal nanoparticles; Raman spectroscopy; Transmission electron microscopy; Nanomagnetics
AbstractNanoparticle labels were used to detect the pathogenic bacteria Staphylococcus aureus. Two different labels were employed. These consisted of nanoparticles based on iron-oxide for magnetic trapping and isolation of the bacteria and surface enhanced Raman spectroscopy (SERS) active gold nanoparticles (AuNPs) coated with 4-mercaptobenzoic acid for the detection and quantification of the bacteria. Both types of nanoparticles were conjugated with single domain antibodies that selectively bind to protein A on the surface of S. aureus. Transmission electron microscopy (TEM) image analysis showed the iron-oxide nanoparticles to have magnetic core diameters of 20 nm ± 3 nm and the gold nanoparticles to be 51 nm ± 2 nm in diameter. A response curve was obtained displaying a logarithmic dependency between the 1073 cm -1 peak intensity in the SERS spectra of the AuNPs and the concentration of S. aureus cells in the initial sample. The overall detection limit was estimated to be 1 S. aureus cell mL-1 in less than 10 min without culturing. © 2013 The Royal Society of Chemistry.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270950
Export citationExport as RIS
Report a correctionReport a correction
Record identifier44f8758e-881a-464b-9268-280a78a6cbf4
Record created2014-02-18
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)