Oxygen plasma treatment of polystyrene and zeonor: substrates for adhesion of patterned cells

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DOIResolve DOI: http://doi.org/10.1021/la9001972
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TypeArticle
Journal titleLangmuir
Volume25
Issue12
Pages71697176; # of pages: 7
AbstractPlastic substrates made of polystyrene (PS) and Zeonor 1060R were treated with oxygen plasma to introduce polar groups (e.g., carbonyl and carboxylic acid) at the surface that render these materials hydrophilic and promote patterned adhesion of HeLa cells. Resultant surfaces were characterized using contact angle goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) to monitor changes in wettability, nanoscale topography, and chemical composition. Biocompatibility of the plastic surfaces was verified through fluorescence microscopy using three fluorophores, Alexa Fluor 488 conjugated to Annexin V, Hoechst 33258, and propidium iodide, indicating cells that undergo apoptosis and necrosis, respectively. The best cell growth was observed on PS treated at 5 W/sccm, for which the viability of adhering HeLa cells exceeded 90%. Patterning was accomplished using an elastomeric microcapillary system (μCS) made of poly(dimethylsiloxane) (PDMS) that consisted of a set of parallel channels to align cells in linear fashion. Densely populated bands were obtained on substrates of both plastic materials when the culture medium contained >2 × 105 cells/mL.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute
Peer reviewedYes
NRC number53852
NPARC number16606878
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Record identifier459bf1a3-c8c0-4b47-9dfb-976b088cb416
Record created2011-01-12
Record modified2016-05-09
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