Soil bacteria and archaea found in long-term corn (zea mays L.) agroecosystems in Quebec, Canada

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DOIResolve DOI: http://doi.org/10.4141/CJSS2012-040
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TypeArticle
Journal titleCanadian Journal of Soil Science
ISSN0008-4271
Volume93
Issue1
Pages4557; # of pages: 13
Subjectagricultural ecosystem; assessment method; bacterium; biomass; cell organelle; conservation tillage; fluorescence; maize; phylogeny; polymerase chain reaction; soil microorganism; species diversity; Canada; Quebec [Canada]; Actinobacteria; Archaea; Bacteria (microorganisms); Crenarchaeota; Firmicutes; Fungi; Proteobacteria; Zea mays
AbstractThe soil microbial community controls all biological processes in soils and is considered a good indicator of general soil health. Assessment of the microbial community in intensively cropped soils that are under reduced tillage management is especially important because the microbes are the primary decomposers of the high residue input in such systems. We investigated the microbial biomass and diversity of bacteria and archaea in a sandy-loam Dystric Gleysol from a long-term (15 yr) corn (Zea mays L.) agroecosystem in Quebec, Canada, under conventional (CT), reduced tillage (RT), and no tillage (NT) and two residue inputs (high level: + R and low level: - R). Analysis included microbial biomass C and N (MBC, MBN), catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH) and 5-(4, 6-dichlorotriazinyl) amino fluorescein hydrochloride (DTAF) cell counts, 16S rRNA polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and an archaeal clone library. The PCR-DGGE analysis identified Proteobacteria, Actinobacteria and Firmicutes as dominant groups in all tillage and residue management treatments. The archaeal group was diverse, with most individuals identified as belonging to the Crenarchaeota phylum. We also detected soil archaea belonging to the newly proposed phylum Thaumarchaeota, the chemolithoautotrophic ammonia-oxidizing archaeota, in a corn agroecosystem in Quebec, Canada. Microbial biomass increased in the +R treatment according to MBC concentration and direct cell counts. Considering results from the CARD-FISH counts (bacterial and archaeal cell counts without fungal cells) and from MBC results (all microbial biomass including fungi) we concluded the likelihood of greater fungal biomass in the NT plots.
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270652
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Record identifier45ad0f5d-db29-4229-9fa4-e2ec4a9469b8
Record created2014-02-17
Record modified2016-05-09
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