Enzymatic hydrolysis of the esterfied diarrhetic shellfish poisoning toxins and pectenotoxins

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DOIResolve DOI: http://doi.org/10.1007/s00216-007-1489-3
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
Volume389
Issue1
Pages335342; # of pages: 8
SubjectDiarrhetic shellfish poisoning (DSP); Okadaic acid; Dinophysistoxins; Pectenotoxins; Esters; Liquid chromatography–mass spectrometry; Enzymatic hydrolysis
AbstractOkadaic acid (OA) and dinophysistoxins-1 and -2 (DTX1, DTX2), the toxins responsible for incidents of diarrhetic shellfish poisoning (DSP), can occur as complex mixtures of ester derivatives in both plankton and shellfish. Alkaline hydrolysis is usually employed to release parent OA/DTX toxins, and analyses are conducted before and after hydrolysis to determine the concentrations of nonesterified and esterified toxins. Recent research has shown that other toxins, including pectenotoxins and spirolides, can also exist as esters in shellfish, but these toxins cannot survive alkaline hydrolysis. A promising alternative approach is enzymatic hydrolysis. In this study, two enzymatic methods were developed for the hydrolysis of 7-O-acyl esters, “DTX3,” and the carboxylate esters of OA, “diol-esters.” Porcine pancreatic lipase induced complete conversion of DTX3 to OA and DTXs within one hour for reference solutions. The presence of mussel tissue matrix reduced the rate of hydrolysis, but an optimized lipase concentration resulted in greater than 95% conversion within four hours. OA-diol-ester was hydrolyzed by porcine liver esterase and was completely converted to OA in less than 30 min, even in the presence of mussel tissue matrix. Esters and OA/DTX toxins were all monitored by LC–MS. Further experiments with pectenotoxin esters indicated that enzymatic hydrolysis could also be applied to esters of other toxins. Enzymatic hydrolysis has excellent potential as an alternative to the conventional alkaline hydrolysis procedure used in the preparation of shellfish samples for the analysis of toxins.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NRC number42735
1293
NPARC number3538291
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Record identifier466688ae-095a-495e-9669-f37b4bea35b0
Record created2009-03-01
Record modified2016-05-09
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