Cell cycle arrest during S or M phase generates polarized growth via distinct signals in Candida albicans

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DOIResolve DOI: http://doi.org/10.1111/j.1365-2958.2005.04727.x
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TypeArticle
Journal titleMolecular Microbiology
ISSN0950-382X
Volume57
Issue4
Pages942959; # of pages: 18
SubjectCandida; candida albicans; cell cycle; Dna; gene expression; mitosis; pha; phenotype; yeast; cell cycle proteins; cell nucleus division; cell polarity; fungal proteins; gene expression profiling; gene expression regulation, fungal; hydroxyurea; mitosis; oligonucleotide array sequence analysis; S phase; transcription, genetic
AbstractTreatments that perturb DNA synthesis or mitosis will activate checkpoints that prevent cell cycle progression and cell proliferation. In yeast-form cells of the fungal pathogen Candida albicans, exposure to hydroxyurea (HU) or shutting off expression of the polo-like kinase CaCDC5 blocked nuclear division and spindle elongation, but activated a highly polarized growth mode. We have used transcription profiling both to characterize the initiation and progression of this polar growth pattern and to determine how cell elongation may be linked to the cell cycle in C. albicans. Different gene expression patterns during early stages of cell elongation support the concept that CaCdc5p-depleted and HU-exposed cells were blocked at different stages of the cell cycle, and suggest that different signals may generate the common polarized growth phenotype. Consistent with this, BUB2 expression was modulated in CaCdc5p-depleted cells, and absence of BUB2 prevented the maintenance of cell polarization, resulting in multibudded, pseudohyphal cells with constrictions. In contrast, HU-induced filaments did not modulate or require BUB2, but were dependent on the GTPase Ras1p. However, at later stages of cell elongation, transcription profiles were more similar, and comparisons with serum-induced hyphae revealed that the cell cycle-arrested filaments expressed several targets of the hyphal signalling pathways. Thus, arresting the yeast cell cycle in S or M phase generates a polarized growth pattern through different mechanisms in C. albicans, and maintenance of the polar growth mode can ultimately lead to the expression of hyphal-associated cell wall and virulence-related factors, in the absence of any external stimuli
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
Identifier10426590
NRC number47467
NPARC number3539455
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Record identifier4c666433-0322-4635-bd3e-e95b3598c8fa
Record created2009-03-01
Record modified2016-05-09
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