Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor

Download
  1. Get@NRC: Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s00425-011-1573-y
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titlePlanta
ISSN0032-0935
1432-2048
Volume235
Issue6
Pages12711288; # of pages: 18
SubjectAnnexin; Arabidopsis; Proteomics; Sacred lotus; Seed thermotolerance; Seed vigor
AbstractAnnexins are multifunctional proteins characterized by their capacity to bind calcium ions and negatively charged lipids. Although there is increasing evidence implicating their importance in plant stress responses, their functions in seeds remain to be further studied. In this study, we identified a heat-induced annexin, NnANN1, from the embryonic axes of sacred lotus (Nelumbo nucifera Gaertn.) using comparative proteomics approach. Moreover, the expression of NnANN1 increased considerably in response to high-temperature treatment. Quantitative real-time PCR (qRT-PCR) revealed that the transcripts of NnANN1 were detected predominantly during seed development and germination in sacred lotus, implicating a role for NnANN1 in plant seeds. Ectopic expression of NnANN1 in Arabidopsis resulted in enhanced tolerance to heat stress in transgenic seeds. In addition, compared to the wild-type seeds, transgenic seeds ectopically expressing NnANN1 exhibited improved resistance to accelerated aging treatment used for assessing seed vigor. Furthermore, transgenic seeds showed enhanced peroxidase activities, accompanied with reduced lipid peroxidation and reduced ROS release levels compared to the wild-type seeds. Taken together, these results indicate that NnANN1 plays an important role in seed thermotolerance and germination vigor.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number55458
NPARC number21268373
Export citationExport as RIS
Report a correctionReport a correction
Record identifier4cf71c2f-bbac-47db-88de-cbfc3bf71d9b
Record created2013-07-04
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)