Structure of the L2 lipopolysaccharide core oligosaccharides of Neisseria meningitidis

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TypeArticle
Journal titleJournal Of Biological Chemistry
Volume267
Issue2
Pages922925; # of pages: 4
Subject1H-NMR; ACID; assignment; ASSIGNMENTS; Canada; Carbohydrate Conformation; Carbohydrate Sequence; Chromatography,Liquid; COMPONENTS; core oligosaccharide; Hydrolysis; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Methylation; Molecular Sequence Data; Neisseria meningitidis; NMR; NUCLEAR; Oligosaccharides; OVERHAUSER; SEROTYPE; STRUCTURAL; structure; SUBSTITUENT; Support,Non-U.S.Gov't; technique; triantennary
AbstractThree different oligosaccharides were identified following mild acid hydrolysis of the lipopolysaccharide obtained from Neisseria meningitidis serotype 2 and their structures elucidated by combined chemical and physical techniques. The use of 500 MHz 1H nmr in both one- and two-dimensional modes as well as nuclear Overhauser effect experiments were employed. To assist in the structural assignments the oligosaccharides were also degraded by chemical and enzymatic procedures to smaller fragments. The oligosaccharides were all triantennary nonasaccharides in which the longest antenna terminates in lacto-N-neotetraose. Two of the nonasaccharides (major components), not separable by column chromatography, were distinguishable only by their different patterns of phosphorylethanolamine substitution and the third minor component by the absence of this substituent
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberGAMIAN1992
NPARC number9361125
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Record identifier4d8e456c-c165-47c2-bbde-a0beaedcd258
Record created2009-07-10
Record modified2017-03-23
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