Effect of cholesterol on the biophysical and physiological properties of a clinical pulmonary surfactant

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DOIResolve DOI: http://doi.org/10.1529/biophysj.106.099762
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TypeArticle
Journal titleBiophysical Journal
ISSN0006-3495
Volume93
Issue4
Pages13911401; # of pages: 11
AbstractPulmonary surfactant is a complex mixture of lipids and proteins that forms a surface-active film at the air-water interface of alveoli capable of reducing surface tension to near 0 mN/m. The role of cholesterol, the major neutral lipid component of pulmonary surfactant, remains uncertain. We studied the physiological effect of cholesterol by monitoring blood oxygenation levels of surfactant-deficient rats treated or not treated with bovine lipid extract surfactant (BLES) containing zero or physiological amounts of cholesterol. Our results indicate no significant difference between BLES and BLES containing cholesterol immediately after treatment; however, during ventilation, BLES-treated animals maintained higher PaO2 values compared to BLES + cholesterol-treated animals. We used a captive bubble tensiometer to show that physiological amounts of cholesterol do not have a detrimental effect on the surface activity of BLES at 37°C. The effect of cholesterol on topography and lateral organization of BLES Langmuir-Blodgett films was also investigated using atomic force microscopy. Our data indicate that cholesterol induces the formation of domains within liquid-ordered domains (Lo). We used time-of-flight-secondary ion mass spectrometry and principal component analysis to show that cholesterol is concentrated in the Lo phase, where it induces structural changes.
Publication date
LanguageEnglish
AffiliationNRC National Institute for Nanotechnology; National Research Council Canada
Peer reviewedYes
NRC number454
NPARC number8926369
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Record identifier4dea0520-09cd-47e4-9afb-ad3e29d4c7c1
Record created2009-04-23
Record modified2016-05-09
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