Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics

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DOIResolve DOI: http://doi.org/10.1021/cr5000943
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TypeArticle
Journal titleChemical Reviews
ISSN0009-2665
1520-6890
Volume114
Issue21
Pages1108311130; # of pages: 48
SubjectAntibodies; Biomolecules; Chemical detection; Curing; Nucleic acids; Opitical properties; Bioanalytical platforms; Biological recognition; Curing condition; Electrode material; Fast response time; High sensitivity; Signal transducers; Variable conditions; Enzyme immobilization
AbstractBiosensing and diagnostic platforms with high sensitivity, specificity, and fast response time are based on immobilized biomolecules such as antibodies (Abs), aptamers, enzymes, nucleic acids, receptors, and whole cells for the detection of target analytes. Such sensing biomolecules should be bound to the surface of a signal transducer with a required specific chemical, electrical, or optical property. The biological recognition event generates a quantifiable signal, which is equated to the amount or concentration of the analyte. APTES can be deposited on solid materials, electrode materials, nanomaterials, and nanocomposites under variable conditions of concentration, solvent, temperature, and time. In addition, curing conditions such as air/heat drying might be necessary depending upon the intended application. Pertinent information on the thickness, morphology, and conformation of the APTES layer reported in the literature is often different and conflicting.
Publication date
LanguageEnglish
AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberNRC-ACRD-56084
NPARC number21273796
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Record identifier4e64096b-57bc-477e-9d1b-ddb753d9f2cf
Record created2015-01-22
Record modified2016-05-09
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