Pasteurella multocida heddleston serovar 3 and 4 strains share a common lipopolysaccharide biosynthesis locus but display both inter- and intrastrain lipopolysaccharide heterogeneity

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DOIResolve DOI: http://doi.org/10.1128/JB.00779-13
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TypeArticle
Journal titleJournal of Bacteriology
ISSN0021-9193
Volume195
Issue21
Pages48544864; # of pages: 11
Subjectbacterium lipopolysaccharide; galactosyltransferase; glycosyltransferase; article; bacterial growth; bacterium isolation; biosynthesis; carbohydrate analysis; controlled study; crystal structure; DNA sequence; electrospray mass spectrometry; Escherichia coli; gene locus; gene mutation; genotype; missense mutation; mutagenesis; nonhuman; nonsense mutation; nuclear magnetic resonance spectroscopy; nucleotide sequence; Pasteurella multocida; priority journal; promoter region; serotype
AbstractPasteurella multocida is a Gram-negative multispecies pathogen and the causative agent of fowl cholera, a serious disease of poultry which can present in both acute and chronic forms. The major outer membrane component lipopolysaccharide (LPS) is both an important virulence factor and a major immunogen. Our previous studies determined the LPS structures expressed by different P. multocida strains and revealed that a number of strains belonging to different serovars contain the same LPS biosynthesis locus but express different LPS structures due to mutations within glycosyltransferase genes. In this study, we report the full LPS structure of the serovar 4 type strain, P1662, and reveal that it shares the same LPS outer core biosynthesis locus, L3, with the serovar 3 strains P1059 and Pm70. Using directed mutagenesis, the role of each glycosyltransferase gene in LPS outer core assembly was determined. LPS structural analysis of 23 Australian field isolates that contain the L3 locus revealed that at least six different LPS outer core structures can be produced as a result of mutations within the LPS glycosyltransferase genes. Moreover, some field isolates produce multiple but related LPS glycoforms simultaneously, and three LPS outer core structures are remarkably similar to the globo series of vertebrate glycosphingolipids. Our in-depth analysis showing the genetics and full range of P. multocida lipopolysaccharide structures will facilitate the improvement of typing systems and the prediction of the protective efficacy of vaccines. © 2013, American Society for Microbiology.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269906
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Record identifier51877006-391d-4266-8d02-4bf699f69b80
Record created2013-12-13
Record modified2016-05-09
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