The identification a novel gene required for lipopolysaccharide biosynthesis by Haemophilus influenzae RM7004, using transposon Tn916 mutagenesis

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TypeArticle
Journal titleFEMS Microbiol Lett.
Volume145
Issue3
Pages325331; # of pages: 7
SubjectAntibodies,Monoclonal; Bacterial Proteins; Bacteriological Techniques; Base Sequence; Blotting,Southern; Chromosomes,Bacterial; DNA Transposable Elements; DNA,Bacterial; Enhancer Elements (Genetics); Escherichia coli; Escherichia-coli; Genes,Bacterial; Genetic Screening; Haemophilus; Haemophilus influenzae; Lipopolysaccharide; Lipopolysaccharides; Molecular Sequence Data; Mutagenesis; Plasmids; Polymerase Chain Reaction; Restriction Mapping; Sequence Analysis,DNA; Sequence Homology,Amino Acid; Support,Non-U.S.Gov't
AbstractMutagenesis with the transposon Tn916 was used as a strategy to identify genes required for synthesis of the Gal alpha (1-4) beta Gal component of Haemophilus influenzae strain RM7004 lipopolysaccharide. Insertion of Tn916 into an open reading frame (ORF) encoding a protein with 75% homology to the Escherichia coli methionine related protein (Mrp) is described. Mutations in mrp resulted in loss of reactivity with monoclonal antibody (mAb) 4C4, which recognises Gal alpha (1-4) beta Gal, and expression of LPS with a different electrophoretic profile to that of wild-type RM7004. An unexpected feature of this mutation was that it appeared to influence the number of copies of 5'-CAAT-3' present in lic2A, a gene which is also required for biosynthesis and phase variable expression of the Gal alpha (1-4) beta Gal LPS epitope Type: JOURNAL ARTICLE Language: Eng
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberHIGH1996
NPARC number9389136
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Record identifier521d0779-8c67-4ecc-92bf-ba74284b4a8e
Record created2009-07-10
Record modified2016-05-09
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