A Fourier transform infrared spectroscopic study of the molecular interaction of cholesterol with 1,2-dipalmitoyl-sn-glycero-3-phosphocholine

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DOIResolve DOI: http://doi.org/10.1016/0005-2736(80)90287-4
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TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA) - Biomembranes
ISSN0005-2736
Volume602
Issue1
Pages3244
SubjectCholesterol; Phosphatidylcholine; Fourier transform infrared spectra; Melting phenomenon; Ester bond
AbstractThe temperature dependencies of the infrared spectra of pure and cholesterol-containing multibilayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine were studied using Fourier transform infrared techniques. A comparison of the spectroscopic data showed the retention of a melting phenomenon at 60 mol% cholesterol content, and the retention of some all-trans conformations in the liquid-crystalline phase. It is also demonstrated that at temperatures less than 30°C, the cholesterol-containing 1,2-dipalmitoyl-sn-glycero-3-phosphocholine multibilayers still contain a small amount of pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, packed in an orthorhombic subcell lattice. Spectral changes were found in the absorptions characteristic of the phospholipid head groups. The addition of cholesterol results in changes in the ester bands, and demonstrates the induction by cholesterol of non-equivalent ester conformations.
Publication date
PublisherElsevier B.V.
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number18358
NPARC number23001275
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Record identifier52357d35-424d-4ac9-a70a-c49455b7c9d0
Record created2017-01-13
Record modified2017-01-13
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