Passive All-Optical Force Clamp for High-Resolution Laser Trapping

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DOIResolve DOI: http://doi.org/10.1103/PhysRevLett.95.208102
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TypeArticle
Journal titlePhysical review letters
Volume95
Issue20
Pages2081024; # of pages: 208099
Subjectbiological techniques; biomechanics; DNA; laser beam effects; molecular biophysics; optical feedback; radiation pressure; stability
AbstractOptical traps are useful for studying the effects of forces on single molecules. Feedback-based force clamps are often used to maintain a constant load, but the response time of the feedback limits bandwidth and can introduce instability. We developed a novel force clamp that operates without feedback, taking advantage of the anharmonic region of the trapping potential where the differential stiffness vanishes. We demonstrate the utility of such a force clamp by measuring the unfolding of DNA hairpins and the effect of trap stiffness on opening distance and transition rates.
Publication date
AffiliationNational Research Council Canada; National Institute for Nanotechnology
Peer reviewedNo
NPARC number12338342
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Record identifier52b3e760-b457-41c5-8107-61969b6cd4f9
Record created2009-09-10
Record modified2016-05-09
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