Solution conformation of alpha D(1-3)- and alpha D(1-6)-linked oligomannosides using proton nuclear magnetic resonance: Biochemistry

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TypeArticle
Journal titleBiochemistry
Volume22
Issue6
Pages13621368; # of pages: 7
Subject13C; 1H; ALPHA; ASPARAGINE-LINKED; CALCULATION; CALCULATIONS; Carbohydrate Conformation; conformation; CONFORMATIONS; COUPLING; coupling constants; COUPLING-CONSTANTS; crystal; CRYSTAL-STRUCTURE; CRYSTAL-STRUCTURES; ENERGY; energy calculations; glycopeptide; Glycopeptides; Glycosides; LINKAGES; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Mannose; Mannosides; Methods; METHYL; Models,Molecular; N-linked; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; OVERHAUSER; POTENTIAL; PROTON; RELAXATION; RESONANCE; solution; SOLUTION CONFORMATION; Solutions; structure; Support,Non-U.S.Gov't; synthetic; technique; Thermodynamics; UNIT
AbstractThe solution conformations of synthetic methyl mannobiosides and a methyl mannotrioside containing alpha D(1-3) and alpha D(1-6) linkages have been determined through the use of 1H nuclear magnetic resonance techniques, namely, the nuclear Overhauser effect and proton relaxation time measurements. 3J(C,H) coupling constants, obtained from a compound enriched with 13C, were also used. The allowed conformations were found to be in agreement with those determined from potential energy calculations and crystal structures. The methyl mannotrioside is an analogue of a mannotriose unit which occurs naturally in the "core" of asparagine-linked glycopeptides and in an "arm" of high mannose N-linked glycopeptides
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberBRISSON1983I
NPARC number9363346
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Record identifier5303f014-e657-4a26-bf89-f1cec41e5936
Record created2009-07-10
Record modified2016-05-09
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