Quercetin deformable liposome: Preparation and efficacy against ultraviolet B induced skin damages in vitro and in vivo

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DOIResolve DOI: http://doi.org/10.1016/j.jphotobiol.2013.07.014
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TypeArticle
Journal titleJournal of Photochemistry and Photobiology B: Biology
ISSN1011-1344
Volume127
Pages817; # of pages: 10
Subject3,4 methylenedioxyamphetamine; cholesterol; cholic acid; liposome; malonaldehyde; phosphatidylcholine; polysorbate 80; quercetin; reactive oxygen metabolite; sorbitan laurate; animal cell; animal experiment; article; cell membrane; cell protection; cell viability; controlled study; dermatitis; drug delivery system; drug efficacy; drug formulation; drug release; drug solubility; drug synthesis; elasticity; encapsulation; histopathology; in vitro study; in vivo study; keratinocyte; lipid peroxidation; male; nonhuman; oxidative stress; particle size; priority journal; rat; skin defect; skin edema; stratum corneum; ultraviolet B radiation; ultraviolet irradiation; zeta potential
AbstractUltraviolet (UV) radiation has deleterious effects on cells through direct damage to DNA or through increasing generation of reactive oxygen species (ROS). The flavonol quercetin (Qu) provides cellular protection against UV radiation and the current investigation was carried out to develop a deformable liposome formulation of Qu to enhance its delivery into human skin and to improve its anti-UVB effect. The influence of surfactants (including Span 20, Tween 80 and sodium cholate) on the properties of Qu deformable liposomes was investigated. Liposomes composed of Qu, phosphatidylcholine (PC), cholesterol (Chol), and Tween 80 showed high entrapment efficiencies (80.41 ± 4.22%), small particle sizes (132 ± 14 nm), high elasticity (10.48 ± 0.71), and prolonged drug release. The cell viability in UVB-irradiated HaCaT cells increased to 89.89 ± 4.5% at 24 h and 78.8 ± 3.19% at 48 h following treatment with Qu defomable liposomes. The ROS and malondialdehyde (MDA) level were also reduced. The penetration rate was 3.8-fold greater than that of the Qu suspension. Moreover, the edema and inflammation was alleviated by Qu deformable liposomes. These results showed the potential of deformable liposomes to enhance the anti-UVB effects of Qu both in vitro and in vivo. © 2013 Elsevier B.V. All rights reserved.
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LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269779
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Record identifier54320dd5-68fe-4e5b-99f7-abaf9344106d
Record created2013-12-13
Record modified2016-05-09
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