Wild-type egr1/Krox24 promotes and dominant-negative mutants inhibit, pluripotent differentiation of p19 embryonal carcinoma cells

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DOIResolve DOI: http://doi.org/10.1038/sj.onc.1202166
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TypeArticle
Journal titleOncogene
ISSN0950-9232
Volume17
Issue19
Pages24952504
SubjectEC cells; differentiation; Krox24; egr1; transcription factors
AbstractThe zinc-finger transcription factor Krox24 was analysed for its role in differentiation in P19 embryonal carcinoma cells. Reciprocal dominant negative mutants consisting of Krox24 deleted for a crucial region of the zinc-finger domain (DeltaKrox24) or of the zinc-finger region alone (DeltaKrox24Zf) abolished the activation of transcription by Krox24 in P19 cells. Expression of Krox24 led to spontaneous differentiation of P19 cells in a lineage-independent fashion. Krox24 transfected populations, as well as individual clones randomly picked from them, displayed a wide array of diverse morphologies and expressed markers characteristic of a variety of differentiated cells. The dominant negative mutants blocked differentiation of P19 cells. We conclude that expression of Krox24 is sufficient for pluripotent differentiation of embryonal carcinoma cells, and that expression of Krox24 or other egr family members is essential to this process.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
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NPARC number23000925
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Record identifier54a311cd-6f26-4414-a0c3-2658f67fb39f
Record created2016-11-16
Record modified2016-11-16
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