Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy

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DOIResolve DOI: http://doi.org/10.1117/1.3490641
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TypeArticle
Journal titleJournal of Biomedical Optics
Volume16
Issue2
Pages021103-1021103-7
SubjectCoherent anti-Stokes Raman scattering; CARS Microscopy
AbstractWe demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.
Publication date
LanguageEnglish
AffiliationNRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19739656
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Record identifier55562410-d97e-49a5-9ca3-a785df2ffbc4
Record created2012-04-02
Record modified2016-05-09
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