A novel N-linked flagellar glycan from Methanococcus maripaludis

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2009.01.006
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TypeArticle
Journal titleCarbohydrate Research
Volume344
Issue5
Pages648653; # of pages: 6
SubjectArchaea; methanococcus maripaludis; flagellar glycosylation; N-linked tetrasaccharide; structure; NMR; analysis; Canada; chromatography; digestion; glycopeptide; glycopeptides; N-linked; NMR spectroscopy; NMR-spectroscopy; oligosaccharide; peptide; Peptides; Pronase; spectroscopy; strain; unit
AbstractThe archaea Methanococcus maripaludis strain Mm900 produces flagella that are glycosylated with an Nlinked tetrasaccharide. Mass spectrometric analysis of flagellar tryptic peptides identified a number of tryptic glycopeptides carrying a glycan of mass 1036.4 Da, and fragmentation of the glycan oxonium ion indicated that the glycan was a tetrasaccharide. The glycan was purified, following extensive pronase digestion of flagellar filaments, by size-exclusion and anion-exchange chromatography. NMR spectroscopy revealed that the glycan had the following structure: Sug-4-β-ManNAc3NAmA6Thr-4-β-Glc- NAc3NAcA-3-β-GalNAc-Asn where Sug is a novel monosaccharide unit, (5S)-2-acetamido-2,4-dideoxy- 5-O-methyl-α-L-erythro-hexos-5-ulo-1,5-pyranose. This oligosaccharide has significant similarity to the oligosaccharide that was found previously in Methanococcus voltae.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NRC numberKELLY2009
NPARC number15469829
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Record identifier56fa5134-c413-4b66-bc6a-89dd12578afe
Record created2010-08-16
Record modified2016-05-09
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