Transcripts of sulphur metabolic genes are co-ordinately regulated in developing seeds of common bean lacking phaseolin and major lectins

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DOIResolve DOI: http://doi.org/10.1093/jxb/ers280
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Experimental Botany
ISSN0022-0957
Volume63
Issue17
Pages62836295; # of pages: 13
Subjectamino acid; cysteine; drug derivative; lectin; messenger RNA; methionine; o acetylserine; O-acetylserine; phaseolin protein, Phaseolus vulgaris; plant RNA; s methylcysteine; S-methylcysteine; seed storage protein; serine; sulfur; vegetable protein; article; cluster analysis; DNA microarray; gene expression profiling; gene expression regulation; genetics; growth, development and aging; metabolism; Phaseolus; plant seed; Amino Acids; Cluster Analysis; Cysteine; Gene Expression Profiling; Gene Expression Regulation, Plant; Lectins; Methionine; Oligonucleotide Array Sequence Analysis; Phaseolus; Plant Proteins; RNA, Messenger; RNA, Plant; Seed Storage Proteins; Seeds; Serine; Sulfur; Phaseolus vulgaris
AbstractThe lack of phaseolin and phytohaemagglutinin in common bean (dry bean, Phaseolus vulgaris) is associated with an increase in total cysteine and methionine concentrations by 70% and 10%, respectively, mainly at the expense of an abundant non-protein amino acid, S-methyl-cysteine. Transcripts were profiled between two genetically related lines differing for this trait at four stages of seed development using a high density microarray designed for common bean. Transcripts of multiple sulphur-rich proteins were elevated, several previously identified by proteomics, including legumin, basic 7S globulin, albumin-2, defensin, albumin-1, the Bowman-Birk type proteinase inhibitor, the double-headed trypsin inhibitor, and the Kunitz trypsin inhibitor. A co-ordinated regulation of transcripts coding for sulphate transporters, sulphate assimilatory enzymes, serine acetyltransferases, cystathionine β-lyase, homocysteine S-methyltransferase and methionine gamma-lyase was associated with changes in cysteine and methionine concentrations. Differential gene expression of sulphur-rich proteins preceded that of sulphur metabolic enzymes, suggesting a regulation by demand from the protein sink. Up-regulation of SERAT1;1 and-1;2 expression revealed an activation of cytosolic O-acetylserine biosynthesis. Down-regulation of SERAT2;1 suggested that cysteine and S-methyl-cysteine biosynthesis may be spatially separated in different subcellular compartments. Analysis of free amino acid profiles indicated that enhanced cysteine biosynthesis was correlated with a depletion of O-acetylserine. These results contribute to our understanding of the regulation of sulphur metabolism in developing seed in response to a change in the composition of endogenous proteins. © 2012 The Author.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Plant Biotechnology Institute (PBI-IBP)
Peer reviewedYes
NPARC number21269172
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Record identifier571a086b-3ed3-4e98-8ea3-21681e385e9a
Record created2013-12-12
Record modified2016-05-09
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