Structure of the L5 lipopolysaccharide core oligosaccharides of Neisseria meningitidis: J.Biol.Chem.

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TypeArticle
Journal titleJournal Of Biological Chemistry
Volume265
Issue13
Pages72437247; # of pages: 5
Subject1H; 1H-NMR; ACID; analysis; assignment; ASSIGNMENTS; Canada; Carbohydrate Conformation; Carbohydrate Sequence; chemical; core oligosaccharide; DECASACCHARIDE; Hydrolysis; isolation & purification; LIPOPOLYSACCHARIDE; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Methylation; Molecular Sequence Data; Neisseria; Neisseria meningitidis; NMR; NUCLEAR; oligosaccharide; Oligosaccharides; one-dimensional; OVERHAUSER; SEROTYPE; STRUCTURAL; structure; Support,Non-U.S.Gov't; technique; triantennary; UNIT
AbstractThree different oligosaccharides were isolated by mild acid hydrolysis of the lipopolysaccharides, obtained from Neisseria meningitidis serotype 5, and their structures were elucidated by combined chemical and physical techniques. The use of 500-MHz 1H NMR in both one-dimensional and two-dimensional modes as well as nuclear Overhauser effect experiments were employed. To assist in the structural assignments the purified oligosaccharides were also degraded by chemical and enzymatic procedures to smaller fragments. The largest of the three original oligosaccharides is a triantennary partially O-acetylated decasaccharide in which the largest antenna terminates in a lacto-N-neotetraose unit. The smaller oligosaccharides (heptasaccharide and octasaccharide) except for terminal glycose deletions from the longest antenna are structural replicas of the larger
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMICHON1990
NPARC number9358584
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Record identifier573784bc-fb11-49da-8af2-6bd45f039dd9
Record created2009-07-10
Record modified2016-05-09
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