Spatial-spectral coupling in hyperspectral CARS microscopy image formation

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DOIResolve DOI: http://doi.org/10.1117/12.2004217
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TypeArticle
Proceedings titleProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 5 February 2013 through 7 February 2013, San Francisco, CA
ISSN1605-7422
ISBN9780819493583
Volume8589
Article number85890T
SubjectCars microscopies; Coherence imaging; Coherent anti-Stokes Raman scattering microscopy; Kramers-Kronig transformation; Nonlinear microscopy; Numerical calculation; Three dimensional images; Three dimensional space; Coherent scattering; Image acquisition; Three dimensional; Time domain analysis; Raman spectroscopy
AbstractHyperspectral coherent anti-Stokes Raman scattering (CARS) microscopy has provided an imaging tool for extraction of 3-dimensional volumetric information, as well as chemically-sensitive spectral information. These techniques have been used in a variety of different domains including biophysics, geology, and material science. The measured CARS spectrum results from interference between the Raman response of the sample and a non-resonant background. We have collected four dimensional data sets (three spatial dimensions, plus spectra) and extracted Raman response from the CARS spectrum using a Kramers-Kronig transformation. However, the three dimensional images formed by a CARS microscope are distorted by interference, some of which arises because of the Gouy phase shift. This type of interference comes from the axial position of the Raman resonant object in the laser focus. We studied how the Gouy phase manifests itself in the spectral domain by investigating microscopic diamonds and nitrobenzene droplets in a CARS microscope. Through experimental results and numerical calculation using finite-diference time-domain (FDTD) methods, we were able to demonstrate the relationship between the spatial configuration of the sample and the CARS spectral response in three dimensional space. © 2013 Copyright SPIE.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270531
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Record identifier57a4a789-7d7a-4cb0-bcb8-e46eb6d01982
Record created2014-02-17
Record modified2016-05-09
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