Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene

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DOIResolve DOI: http://doi.org/10.1016/j.talanta.2012.05.014
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TypeArticle
Journal titleTalanta
ISSN00399140
Volume99
Pages2228; # of pages: 7
SubjectGraphene; Glucose biosensor; APTES; Mediatorless; Amperometric; Glucose oxidase
AbstractA mediatorless glucose biosensor was developed by the immobilization of glucose oxidase (GOx) to graphene-functionalized glassy carbon electrode (GCE). The surface of GCE was functionalized with graphene by incubating it with graphene dispersed in 3-aminopropyltriethoxysilane (APTES), which acted both as a dispersion agent for graphene and as an amine surface modification agent for GCE and graphene. This was followed by the covalent binding of GOx to graphene-functionalized GCE using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) based crosslinking. Graphene provided signal enhancement by providing greater surface area for GOx binding, while APTES-functionalization led to a higher GOx immobilization density by providing free amino groups for crosslinking. The developed biosensor used a redox potential of −0.45 V (vs. Ag/AgCl) for detecting glucose in the diabetic pathophysiological range 0.5–32 mM. There was no interference from endogenous electroactive substances and drug metabolites. The developed biosensor was further validated for detecting blood glucose in commercial artificial blood glucose linearity standards in the range 1.4–27.9 mM. Therefore, it is ideal for diabetic blood glucose monitoring. The developed bioanalytical procedure for preparation of GOx-bound graphene-functionalized GCEs had high production reproducibility and high storage stability, which is appropriate for the commercial mass production of enzyme-bound electrodes.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
IdentifierS0039914012003918
NPARC number21268739
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Record identifier57b794fb-d8f8-4c7e-930e-8219caaa7cef
Record created2013-11-12
Record modified2016-05-09
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