Capillary electrophoresis mass spectrometry for the characterization of O-acetylated N-glycans from fish serum

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DOIResolve DOI: http://doi.org/10.1021/ac301889k
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TypeArticle
Journal titleAnalytical Chemistry
ISSN0003-2700
1520-6882
Volume84
Issue20
Pages87568762; # of pages: 7
AbstractO-Acetylated N-glycans from fish serum of Atlantic salmon (Salmo salar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS) were separated to obtain a CE-LIF profile of the complex glycan mixture, and the profile concurs with that obtained by using electrospray mass spectrometry. The identity of the APTS-labeled glycans was confirmed by CE–MS. The same glycans can be identified also in their native state by CE–MS without derivatization. The structural variations of O-acetylated sialic acid isomers in fish serum glycans are investigated by CE–MS/MS. Selected ion monitoring provided useful structural information of the underivatized glycans from fragmentation spectra. New complex fish serum glycans that are not reported previously were observed and characterized. These methods may be useful not only for the characterization of acetylation of complex glycans but also to study other types of glycan modifications, as well as to allow determination of overall glycan composition in glycoproteins.
Publication date
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NPARC number21268858
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Record identifier581586a7-a076-4e6d-ae94-32cfa1f9264d
Record created2013-11-15
Record modified2016-05-09
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