Dynamic manganese-enhanced magnetic resonance imaging can detect chronic cryoinjury-induced infarction in pig hearts in vivo

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DOIResolve DOI: http://doi.org/10.1002/cmmi.438
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TypeArticle
Journal titleContrast Media & Molecular Imaging
Volume6
Issue6
Pages426436; # of pages: 11
Subjectmanganese chloride; Gd-DTPA; T1-weighted magnetic resonance imaging; pig heart in vivo; cryoinjury-induced myocardial infarction; blood pressure; heart rate; triphenyltetrazolium chloride staining
AbstractThe purpose was to investigate whether MnCl2 can serve as an MRI contrast agent to detect chronic cryoinjury infarction in pigs in vivo and whether MnCl2 causes significant hemodynamic disturbances. Hearts were subjected to a topical 2 min cryothermia to establish myocardial infarction (MI). Thereafter GdDTPA-enhanced MRI was performed at 0, 1, 2 and 3 weeks using a 3 T scanner. Four weeks post-cryoinjury the pigs underwent in vivo Mn-enhanced magnetic resonance imaging (MEMRI). MnCl2 (70 μmol/kg, 14 min) was infused i.v. intermittently (n = 4) or continuously (n = 5) and T1-weighted images were acquired every 2 min simultaneously recording heart rate and arterial blood pressure. Either infusion scheme led to an immediate increment in MR signal intensity (SI) within the left ventricular (LV) blood pool and LV normal and cryoinjured myocardium, which reached a maximum at the end of infusion. No significant difference was observed between the normal and cryoinjured myocardium. After infusion termination, SI decreased faster within the LV blood pool and the MI, as compared with the normal myocardium in either group, resulting in significant contrast between the MI and normal tissue (intermittent: 18 ± 7 vs 49 ± 13%, p = 0.002; continuous: 19 ± 8 vs 36 ± 9%, p = 0.004). Infarction sizes were similar in Mn2+- and GdDTPA-enhanced images at 4 and 3 weeks post injury, respectively. Thus, in vivo MEMRI differentiated infarcted from normal myocardium in pig hearts subjected to 4-week cryoinjury. Compared with intermittent infusion, continuous infusion minimized hemodynamic fluctuations.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number19703839
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Record identifier586f1ffb-445a-42a8-a3f4-37cbfc1b9fd2
Record created2012-03-28
Record modified2016-05-09
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