Cyclohexanol biodegradation genes: a pathway of opportunities

DOIResolve DOI: http://doi.org/10.1021/bk-2003-0840.ch006
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TypeBook Chapter
Book titleBiocatalysis in Polymer Science
ACS Symposium Series
Volume840
Pages8092; # of pages: 13
AbstractWe have now determined the complete gene sequence of the cyclohexanol (chn) degradation pathway in Acinetobacter sp. NCIMB 9871 as well as the putative genes for the β-oxidation of adipic acid to acetyl-CoA and succinyl-CoA. In addition, a new insertion sequence, potentially useful in strain characterization, was identified. Knowledge of the nucleotide sequence of the chn genes was used to construct clones of Escherichia coli that would overproduce the requisite biocatalysts: a flavin monooxygenase (ChnB; cyclohexanone 1,2-monooxygenase [CHMO]), a ring-opening hydrolase (ChnC; ε-caprolactone hydrolase) and three oxido-reductases (ChnA, cyclohexanol dehydrogenase; ChnD, 6-hydroxyhexanoate dehydrogenase; and ChnE, 6-oxohexanoate dehydrogenase). Besides the well known application of CHMO as a Baeyer-Villiger biocatalyst that carries out stereoselective oxidations of a wide variety of ketones to the corresponding lactones, potential applications of the Chn biocatalysts in the development of "green" bioprocesses such as an "envirocompatible" synthesis of adipic acid are discussed.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC numberBCENV018
NPARC number3540222
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Record identifier5971f303-e7a8-403c-b483-3c50654d66ed
Record created2009-03-01
Record modified2016-05-09
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