FT-IR spectroscopy of methylmercury-exposed mouse lung

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DOIResolve DOI: http://doi.org/10.1007/BF00925716
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TypeArticle
Journal titleMolecular and Cellular Biochemistry
ISSN0300-8177
1573-4919
Volume145
Issue1
Pages7579; # of pages: 5
Subjectinfrared spectroscopy; methylmercury; mouse lung; BAL; lung surfactant; OM
AbstractInfrared spectrocopy which has traditionally been utilized by chemists and physicists for characterization and identification of the structural properties of chemical compounds is now becoming more relevant as a biodiagnostic tool. Recent reports suggest that arthritis and Alzheimer's disease can be diagnosed by using this technique. Changes associated with these diseases diagnosable with this technique are generally overt. In this study we have used ‘Fourier transform infrared spectroscopy’ (FT-IR) to analyze subtle changes in composition and structure of lipids and proteins in lung tissue, bronchoal veolar lavage and purified lamellar body fraction of mice exposed to methylmercury. Infrared measurements were made in attenuated total reflection mode using the Split PeaTM (Harrick Scientific Corporation, USA). Mice were treated with 4 doses of methylmercuric chloride (15 mg/kg body weight/dose), and control animals received an equivalent volume of physiological saline. Comparison of the control and experimental spectra revaled alterations in the intensities and frequencies of vibrational modes of lipids following methylmercury exposure. Results indicate that FT-IR spectral analyses may be a valuable tool for detecting subtle variations in biological components associated with drug exposure to lungs and, in particular may be very useful for assessing changes in bronchoalveolar lavage.
Publication date
PublisherKluwer Academic Publishers
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number174
NPARC number9147833
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Record identifier59823ecb-b452-4452-b325-ec8f1590b0a0
Record created2009-06-25
Record modified2016-11-28
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