Isolation of hyaluronic acid from human synovial fluid by pronase digestion and gel-filtration

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DOIResolve DOI: http://doi.org/10.1016/S0008-6215(00)81776-0
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TypeArticle
Journal titleCarbohydrate Research
Volume2
Issue1
Pages4955; # of pages: 7
AbstractThe isolation of hyaluronic acid from human synovial fluid by passage down Sephadex G-200, following sterile digestion with pronase in Tris buffer (pH 7.9) for 8 h at 37°, affords a product without the occurrence of the enzymic or chemical degradation that may accompany other methods of isolation. Use of phosphate buffer, under similar conditions, gives turbid solutions of hyaluronic acid.
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LanguageEnglish
Peer reviewedYes
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NRC numberBARKER1966
NPARC number9381711
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Record identifier598c4d6b-286f-47da-837a-9f5a1d7d3bd5
Record created2009-07-10
Record modified2016-05-09
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