A comparison of the effect of one and two mono-unsaturated acyl chains on the structure of phospholipid bilayers : a high pressure infrared spectroscopic study

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DOIResolve DOI: http://doi.org/10.1016/0009-3084(88)90117-X
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TypeArticle
Journal titleChemistry and Physics of Lipids
ISSN0009-3084
Volume46
Issue2
Pages7987
Subjectlipid bilayer; gel phase; cis unsaturation; mixed-chain phospholipids; high pressure; Fourier transform infrared spectroscopy; (monoenoic lecithins)
AbstractIn order to compare the effect of one and two mono-unsaturated (oleoyl) acyl chains on the structure and packing of phospholipid bilayers, infrared spectra of aqueous dispersions of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and several monoenoic lecithins, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (OPPC), and 1-O-hexadecyl-2-oleoyl-sn-glycero-3-phosphocholine (HOPC), were measured at 28°C as a function of pressure up to 47 kbar. Whereas no pressure-induced correlation-field splitting of the CH₂ scissoring or rocking modes is observed in the spectra of the dimonounsaturated system (DOPC), both of these modes split at high pressure (>8 kbar) in the spectra of the mono-unsaturated systems (POPC, OPPC and HOPC). The observation of this splitting rules out a parallel orientation of the acyl chains in the mono-unsaturated systems. The very weak CH₂ band progression observed in the gel phase spectra of the monoenoic lecithins indicates that the oleoyl chain in these systems is significantly disordered, whereas the strong band progression observed in the gel phase of DOPC suggests that the polymethylene chain segments of the oleoyl chains are in their trans conformation. The absence in the POPC, OPPC or HOPC gel phase spectra of the 2820 and 630 cm⁻¹ bands associated in DOPC with the C−H stretching mode of CH₂ groups adjacent to the double bond, and with the out-of-plane = CH deformation mode, respectively, indicates that the conformation of the oleoyl chain in the region of the cis double bond is different in the mono-unsaturated systems.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NPARC number23001414
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Record identifier5a0754ba-2f94-4253-b9d4-387a2054befd
Record created2017-02-02
Record modified2017-02-02
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