Pentabody-mediated antigen delivery induces antigen-specific mucosal immune response

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DOIResolve DOI: http://doi.org/10.1016/j.molimm.2009.02.007
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TypeArticle
Journal titleMolecular Immunology
Volume46
Issue8-9
Pages17181726; # of pages: 9
SubjectSingle domain antibody; Mucosal immunization; CT; CTB; Animal; Animals; Antibodies; antibody; ANTIGEN; B-SUBUNIT; Canada; Cholera; COMPONENT; COMPONENTS; DOMAIN; EFFICIENT; Escherichia; Escherichia coli; ESCHERICHIA-COLI; Human; Humans; Immunization; Mice; Mixture; MODEL; Role; Serum; SYSTEM; toxicity
AbstractAn efficient immunization system is essential for the development of mucosal vaccine. Cholera toxin (CT) and Escherichia coli heat labile toxin (LT) are among the strongest adjuvants tested in experimental animals but their use in humans has been hindered by their toxicity. On the other hand, the role of their non-toxic B-subunits, CTB or LTB, in enhancing mucosal immune response is not clear.We propose here a novel strategy for the induction of mucosal immune responses. Single domain antibodies (sdAbs) against a model antigen bovine serum albumin (BSA) were raised fromthe antibody repertoire of a llama immunized with BSA, pentamerized by fusing the sdAbs to CTB, generating the so-called pentabodies. These pentabodies were used to deliver the antigen by mixing the two components and administering the mixture to mice intranasally. One construct was equivalent to CT in helping induce mucosal immune response. It was also found that this ability was probably due to its high affinity to BSA, providing some insight into the controversial role of CTB in mucosal immunization: at least for BSA, the model antigen BSA employed in this study, CTB has to be tightly linked to the antigen to have adjuvant/immune-enhancing effect.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NRC numberLI2009
NPARC number15336756
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Record identifier5a0e0655-3e50-466c-ae44-49d6cbe239e9
Record created2010-05-21
Record modified2016-05-09
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