Carbohydrate-antibody interactions by NMR for a 13C-labelled disaccharide ligand

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TypeArticle
Journal titleCanadian Journal of Chemistry
Volume80
Issue8
Pages904907; # of pages: 4
Subject13C; 1H; 1H-NMR; analysis; Antibodies; antibody; binding; carbohydrate; complex; COMPLEXES; DISACCHARIDE; Form; interaction; LIGAND; METHYL; MONOCLONAL-ANTIBODIES; MONOCLONAL-ANTIBODY; NMR; NMR spectroscopy; NMR-spectroscopy; NOE; protein; RESONANCE; RESONANCES; SIGNALS; SPECTRA; SPECTROSCOPY
AbstractIncorporation of a 13C label into a carbohydrate ligand, methyl 3- O -(3,6-dideoxy-alpha-D-xylohexopyranosyl)-2- O -methyl-alpha-D-mannopyranoside permitted by NMR spectroscopy the study of its binding to the Fab from a monoclonal antibody, Se 155-4. The signals of the free and bound form were observed in the 13C spectrum of the carbohydrate-protein complex. The dissociation rate constants were consequently determined by full lineshape analysis of the 13C spectrum. Comparison with simplified analyses relying only on the linewidth of the 1H and 13C signals of the free ligand were made and the justifications of underlying assumptions used in these analyses were discussed. For 1H NMR, the protein resonances were purged with a 13C filter to observe only the ligand resonances and NOEs between the ligand and the protein.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberUHRIN2002
NPARC number9379975
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Record identifier5a2f148f-0842-473c-a0fb-3074b289b7b1
Record created2009-07-10
Record modified2016-05-09
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