Biodegradation of cyclic nitramines by tropical marine sediment bacteria

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DOIResolve DOI: http://doi.org/10.1007/s10295-005-0239-9
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TypeArticle
Journal titleJournal of Industrial Microbiology and Biotechnology
Volume32
Issue6
Pages261267; # of pages: 7
SubjectRDX; HMX; Unexploded ordnance; Biodegradation; Tropical marine sediment
AbstractUndersea deposition of unexploded ordnance (UXO) constitutes a potential source of contamination of marine environments by hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX). The goal of the present study was to determine microbial degradation of RDX and HMX in a tropical marine sediment sampled from a coastal UXO field in the region of Oahu Island in Hawaii. Sediment mixed cultures growing in marine broth 2216 (21°C) anaerobically mineralized 69% or 57% (CO2, 25 days) of the total carbon of [UL-14 C]-RDX (100 mgrM) or [UL-14 C]-HMX (10 mgrM), respectively. As detected by PCR-DGGE, members of gamma-proteobacteria (Halomonas), sulfate-reducing delta-proteobacteria (Desulfovibrio), firmicutes (Clostridium), and fusobacterium appeared to be dominant in RDX-enrichment and/or HMX-enrichment cultures. Among 22 sediment bacterial isolates screened for RDX and HMX biodegradation activity under anaerobic conditions, 5 were positive for RDX and identified as Halomonas (HAW-OC4), Marinobacter (HAW-OC1), Pseudoalteromonas (HAW-OC2 and OC5) and Bacillus (HAW-OC6) by their 16S rRNA genes. Sediment bacteria degraded RDX to N2O and HCHO via the intermediary formation of hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX) and methylenedinitramine. The present findings demonstrate that cyclic nitramine contaminants are likely to be degraded upon release from UXO into tropical marine sediment.
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AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number47236
NPARC number3539586
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Record identifier5b212f4d-a776-4f0a-9dc0-e263e982264e
Record created2009-03-01
Record modified2016-05-09
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