Modular ultrasonic Lysis system for rapid nucleic acid extraction and sample transfer of Bacillus spores

Download
  1. Get@NRC: Modular ultrasonic Lysis system for rapid nucleic acid extraction and sample transfer of Bacillus spores (Opens in a new window)
DOIResolve DOI: http://doi.org/10.4172/2157-2526.1000119
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Bioterrorism & Biodefense
Volume3
Issue3
Pages1000119-11000119-6; # of pages: 6
SubjectBacillus spores; sonication; spore lysis; PCR; Sample transfer
AbstractThis paper describes the design, functioning and use of an ultrasonic modular system intended for rapid extraction and fragmentation of DNA from microbial organisms following sample collection in the field. PCR assessment of the DNA extracts revealed that the system can disrupt Bacillus atrophaeus spores, a simulant for Bacillus anthracis, in less than 1 min, providing a DNA yield equivalent to that of a commercial nucleic acid extraction method. Simulation of the transfer from a contaminated to a secure area confirmed that the sample remained confined within the module while the exterior surface can be decontaminated through immersion in a disinfectant solution.
Publication date
LanguageEnglish
AffiliationMedical Devices; National Research Council Canada
Peer reviewedYes
NRC number140326
NPARC number21268115
Export citationExport as RIS
Report a correctionReport a correction
Record identifier5bd60e26-548a-4bbf-9ae2-dda503318ab6
Record created2013-04-12
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)