Biosensor for arsenite using arsenite oxidase and multiwalled carbon nanotube modified electrodes

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DOIResolve DOI: http://doi.org/10.1021/ac070766i
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TypeArticle
Journal titleAnalytical Chemistry
Volume79
Issue20
Pages78317837; # of pages: 7
Subjectanalysis; Copper; env; Water
AbstractA biosensor for arsenite has been developed using molybdenum-containing arsenite oxidase, prepared from the chemolithoautotroph NT-26 that oxidizes arsenite to arsenate. The enzyme was galvanostatically deposited for 10 min at 10 A onto the active surface of a multiwalled carbon nanotube modified glassy carbon electrode. The resulting biosensor enabled direct electron transfer, i.e., effecting reduction and then reoxidization of the enzyme without an artificial electron-transfer mediator. Arsenite was detected within 10 s at an applied potential of 0.3 V with linearity up to 500 ppb and a detection limit of 1 ppb. The biosensor exhibited excellent reproducibility, 2% at 95% confidence interval for 12 repeated analyses of 25 ppb arsenite. Copper, a severe interfering species commonly found in groundwater, did not interfere, and the biosensor was applicable for repeated analysis of spiked arsenite in tap water, river water, and a commercial mineral water.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49086
NPARC number3538694
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Record identifier5c129064-8e1c-4639-9313-d1e2521bf851
Record created2012-02-23
Record modified2016-05-09
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