Structural determinants defining common stereoselectivity of lipases toward secondary alcohols

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DOIResolve DOI: http://doi.org/10.1139/m95-199
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume41
Issue13
Pages289296; # of pages: 8
Subjectlipases; stereoselectivity; three-dimensional structure; conformational rearrangement; pha
AbstractIn this review we summarize some aspects of the enantiopreference of the lipase from Candida rugosa following structural analysis of complexes of this lipase with two enantiomers of an analog of a tetrahedral intermediate in the hydrolysis of simple esters. The analysis of the molecular basis of the enantiomeric differentiation suggests that these results can be generalized to a large class of lipases and esterases. We also summarize our experiments on identification of the key regions in the lipases from Geotrichum candidum lipase responsible for differentiation between fatty acyl chains.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number38532
NPARC number3538624
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Record identifier5ca0fd4f-48b3-486a-a51e-0a15c475ea3f
Record created2011-09-07
Record modified2016-05-09
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