Effect of experimental contamination with the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine on soil bacterial communities

Download
  1. Get@NRC: Effect of experimental contamination with the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine on soil bacterial communities (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/S0168-6496(02)00403-8
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleFEMS Microbiology Ecology
Volume43
Issue2
Pages255262; # of pages: 8
Subjectenv; Electrophoresis
AbstractThe effect of contamination with the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) on an indigenous soil bacterial community was examined in two uncontaminated loam soil columns possessing native grasses. One column was spiked twice with RDX crystals for a total RDX load of 1000 mg (kg soil)-1. The reduced metabolite of RDX degradation, hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine, was observed in the column leachate, suggesting anaerobic degradation of RDX. Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA from both contaminated and uncontaminated columns produced identical banding patterns which were stable over the course of the experimental period. The bacterial diversity remained high in the contaminated column, as determined by restriction fragment length polymorphism and rarefaction analyses of random 16S rDNA clones. These combined results suggested that long-term exposure to 1000 mg RDX (kg soil)-1 did not produce an observable effect on bacterial diversity or the numerically dominant members of the indigenous soil bacterial community. (c) Biosciences Information Services.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45893
NPARC number3540063
Export citationExport as RIS
Report a correctionReport a correction
Record identifier5d40786a-132f-45ce-84b3-526d21c68140
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)