Geniposide regulates glucose-stimulated insulin secretion possibly through controlling glucose metabolism in INS-1 cells

Download
  1. Get@NRC: Geniposide regulates glucose-stimulated insulin secretion possibly through controlling glucose metabolism in INS-1 cells (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1371/journal.pone.0078315
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titlePLoS ONE
ISSN1932-6203
Volume8
Issue10
Article numbere78315
Subject2 oxoglutaric acid; adenosine triphosphate; geniposide; glucose; pyruvate carboxylase; animal cell; article; cell protection; diabetes mellitus; enzyme activation; enzyme regulation; enzyme synthesis; gluconeogenesis; glucose metabolism; glucose transport; immunoblotting; insulin release; nonhuman; nucleotide sequence; pancreas islet beta cell; protein analysis; protein blood level; protein expression; protein function; rat
AbstractGlucose-stimulated insulin secretion (GSIS) is essential to the control of metabolic fuel homeostasis. The impairment of GSIS is a key element of β-cell failure and one of causes of type 2 diabetes mellitus (T2DM). Although the KATP channel-dependent mechanism of GSIS has been broadly accepted for several decades, it does not fully describe the effects of glucose on insulin secretion. Emerging evidence has suggested that other mechanisms are involved. The present study demonstrated that geniposide enhanced GSIS in response to the stimulation of low or moderately high concentrations of glucose, and promoted glucose uptake and intracellular ATP levels in INS-1 cells. However, in the presence of a high concentration of glucose, geniposide exerted a contrary role on both GSIS and glucose uptake and metabolism. Furthermore, geniposide improved the impairment of GSIS in INS-1 cells challenged with a high concentration of glucose. Further experiments showed that geniposide modulated pyruvate carboxylase expression and the production of intermediates of glucose metabolism. The data collectively suggest that geniposide has potential to prevent or improve the impairment of insulin secretion in β-cells challenged with high concentrations of glucose, likely through pyruvate carboxylase mediated glucose metabolism in β-cells. © 2013 Liu et al.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Aquatic and Crop Resource Development (ACRD-DCRA)
Peer reviewedYes
NPARC number21269936
Export citationExport as RIS
Report a correctionReport a correction
Record identifier5dc68ed3-4220-4397-90d5-e49480f9bbe0
Record created2013-12-13
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)