Metabolism of Hexahydro-1, 3, 5-trinitro-triazine (RDX) through initial reduction to Hexahydro-1-nitroso-3, 5-dinitro-triazine (MNX) followed by denitration by Clostridium bifermentans HAW-1

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DOIResolve DOI: http://doi.org/10.1007/s00253-003-1364-x
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleApplied Microbiology and Biotechnology
Volume63
Issue2
Pages187193; # of pages: 7
Subjectenv; metabolism
AbstractA fast hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-degrading [28.1 micromol h(-1) g (dry weight) cells(-1); biomass, 0.16 g (dry weight) cells(-1)] and strictly anaerobic bacterial strain, HAW-1, was isolated and identified as Clostridium bifermentans using a 16S-rRNA-based method. Based on initial rates, strain HAW-1 transformed RDX to hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine (MNX), hexahydro-1,3-dinitroso-5-nitro-1,3,5-triazine (DNX), and hexahydro-1,3,5-trinitroso-1,3,5-triazine (TNX) with yields of 56, 7.3 and 0.2%, respectively. Complete removal of RDX and its nitroso metabolites produced (%, of total C or N) methanol (MeOH, 23%), formaldehyde (HCHO, 7.4%), carbon dioxide (CO2, 3.0%) and nitrous oxide (N2O, 29.5%) as end products. Under the same conditions, strain HAW-1 transformed MNX separately at a rate of 16.9 micromol h(-1) g (dry weight) cells(-1) and produced DNX (25%) and TNX (0.4%) as transient products. Final MNX transformation products were (%, of total C or N) MeOH (21%), HCHO (2.9%), and N2O (17%). Likewise strain HAW-1 degraded TNX at a rate of 7.5 micromol h(-1) g (dry weight) cells(-1 )to MeOH and HCHO. Furthermore, removal of both RDX and MNX produced nitrite (NO2-) as a transient product, but the nitrite release rate from MNX was quicker than from RDX. Thus, the predominant pathway for RDX degradation is based on initial reduction to MNX followed by denitration and decomposition. The continued sequential reduction to DNX and TNX is only a minor route.
Publication date
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number45951
NPARC number3540082
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Record identifier5e176edd-5919-400e-8fe3-ffce127a3c7f
Record created2009-03-01
Record modified2016-05-09
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