Biodegradation of gasoline by gellan gum-encapsulated bacterial cells

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Journal titleBiotechnology and Bioengineering
Pages175184; # of pages: 10
AbstractEncapsulated cell bioaugmentation is a novel alternative solution to in situ bioremediation of contaminated aquifers. This study was conducted to evaluate the feasibility of such a remediation strategy based on the performance of contaminant. An enriched bacterial consortium, isolated from a gasoline-polluted site, was encapsulated in gellan gum microbeads (16-53 mum diameter). The capacity of the encapsulated cells to degrade gasoline under aerobic conditions was evaluated in comparison with free (non-encapsulated) cells. Encapsulated cells (2.6 mgcells g-1 bead) degraded over 90% gasoline hydrocarbons (initial concentration 50-600 mg L-1) within 5-10 days at 10�C. Equivalent levels of free cells removed comparable amounts of gasoline (initial concentration 50-400 mg L-1) within the same period but required up to 30 days to degrade the highest level of gasoline tested (600 mg L-1). Free cells exhibited a lag phase in biodegradation, which increased from 1 to 5 days with an increase in gasoline concentration (200-600 mg L-1). Encapsulation provided cells with a protective barrier against toxic hydrocarbons, eliminating the adaptation period required by free cells. The reduction of encapsulated cell mass loading from 2.6 to 1.0 mgcells g-1 bead caused a substantial decrease in the extent of biodegradation within a 30-day incubation period. Encapsulated cells dispersed within the porous soil matrix of saturated soil microcosms demonstrated a reduced performance in the removal of gasoline (initial concentrations of 400 and 600 mg L-1), removing 30-50% gasoline hydrocarbons compared to 40-60% by free cells within 21 days of incubation. The results of this study suggest that gellan gum-encapsulated bacterial cells have the potential to be used for biodegradation of gasoline hydrocarbons in aqueous systems. � 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 80: 175-184, 2002.
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AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12328443
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Record identifier5e745600-0e2a-493c-b00f-0bc51d445e1c
Record created2009-09-10
Record modified2016-05-09
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