Oligosaccharide and peptidoglycan of Ganoderma lucidum activate the immune response in human mononuclear cells

Download
  1. Get@NRC: Oligosaccharide and peptidoglycan of Ganoderma lucidum activate the immune response in human mononuclear cells (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/jf3000339
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Agricultural and Food Chemistry
ISSN0021-8561
1520-5118
Volume60
Issue11
Pages28302837; # of pages: 8
SubjectGanoderma lucidum; peptidoglycan; oligosaccharide; glucuronic acid; human mononuclear cells
AbstractThe acid-hydrolyzed fragments of Ganoderma lucidum polysaccharides (GLPS) obtained by Smith degradation were separated by size-exclusion chromatography into two major water-soluble fractions: peptidoglycans (GLPS-SF1) and oligosaccharides (GLPS-SF2). Both fractions induced CD69 in human peripheral blood mononuclear cells (hPB-MNCs), and they displayed distinct immunomodulating properties. GLPS-SF1, with a molecular weight of around 20 kDa, were heterogeneous peptidoglycans composed of glucose/mannose (4:1) that exhibited biological activities with Th1 cytokines IL-12, IL-2, TNF-α, and IFN-γ in hPB-MNCs and stimulated macrophage cytokine expression via Toll-like receptor 4 (TLR4) signaling. For GLPS-SF2, with a molecular weight of around several kilodaltons, its sugar sequence was elucidated by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy as [−α-1,4-Glc–(β-1,4-GlcA)3−]n. This oligosaccharide displayed specific immune property with low monocyte induction, greatly stimulated cell activation and proliferation of NK and T cells. This oligosaccharide isolated from G. lucidum polysaccharides with internal glucuronic acids/glucose repeat unit in a 3:1 ratio may be responsible for the active stimulation of NK and T cells.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number21269035
Export citationExport as RIS
Report a correctionReport a correction
Record identifier5ef5ac32-e053-42f8-9a6a-a82813d51def
Record created2013-12-02
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)