Comparison of different support materials for their capacity to immobilize Mycobacterium austroafricanum IFP 2012 and to adsorb MtBE

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DOIResolve DOI: http://doi.org/10.1016/j.enzmictec.2006.10.040
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TypeArticle
Journal titleEnzyme and Microbial Technology
Volume40
Issue6
Pages15241530; # of pages: 7
SubjectMtBE biodegradation; mycobacterium austroafricanum IFP 2012; immobilizing agent; support material; respiration; adsorption
AbstractA strain of Mycobacterium austroafricanum capable of mineralizing methyl tert-butyl ether (MtBE) as its sole carbon and energy source had previously been isolated from activated sludge. The use of this bacteria in a biobarrier system for the in situ treatment of contaminated groundwater was the ultimate goal of this work. This paper reports the results of initial studies to find an appropriate immobilizing or support material. Several materials (peat, perlite, pozzolan, pumice and granulated activated carbon) were considered. Selection was based on the measurement and comparison of their abilities to immobilize or retain the bacteria, and to adsorb MtBE.The immobilization test was based on monitoring the respiration of bacteria remaining on the material after initial contact times of either 24 or 72 h. O₂ consumption and CO₂ production were measured over a period of time. The study showed that perlite was the best material.Adsorption isotherms for MtBE were constructed for each support. The strength of adsorption coefficient, Kf, in the Freundlich equation was calculated and used as the basis for comparison of their adsorptive capacities. The strength of adsorption was highest for the granulated activated carbon, and the Kf values for the other materials were relatively low and fell within the same range. However, since GAC performed poorly in the immobilization test, perlite was selected as the best support material.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49032
NPARC number3540193
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Record identifier5f7a43c3-7381-4154-b234-ca5169bb0205
Record created2009-03-01
Record modified2016-05-09
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