Prebiotic effects of diet supplemented with the cultivated red seaweed Chondrus crispus or with fructo-oligo-saccharide on host immunity, colonic microbiota and gut microbial metabolites

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DOIResolve DOI: http://doi.org/10.1186/s12906-015-0802-5
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TypeArticle
Journal titleBMC Complementary and Alternative Medicine
ISSN1472-6882
Volume15
Issue1
Article number279
Subjectacid; fructose oligosaccharide; immunoglobulin; prebiotic agent; RNA 16S; animal experiment; ascending colon; Bifidobacterium breve; body weight; Chondrus crispus; Clostridium septicum; enzyme linked immunosorbent assay; feces analysis; gas chromatography; immune response; immunoglobulin blood level; immunomodulation; organ weight; rat; RNA sequence; seaweed; Streptococcus pneumonia
AbstractBackground: Gastrointestinal microbial communities are diverse and are composed of both beneficial and pathogenic groups. Prebiotics, such as digestion-resistant fibers, influence the composition of gut microbiota, and can contribute to the improvement of host health. The red seaweed Chondrus crispus is rich in dietary fiber and oligosaccharides, however its prebiotic potential has not been studied to date. Methods: Prebiotic effects were investigated with weaning rats fed a cultivated C. crispus-supplemented diet. Comparison standards included a fructo-oligo-saccharide (FOS) diet and a basal diet. The colonic microbiome was profiled with a 16S rRNA sequencing-based Phylochip array. Concentrations of short chain fatty acids (SCFAs) in the feacal samples were determined by gas chromatography with a flame ionization detector (GC-FID) analysis. Immunoglobulin levels in the blood plasma were analyzed with an enzyme-linked immunosorbent assay (ELISA). Histo-morphological parameters of the proximal colon tissue were characterized by hematoxylin and eosin (H&E) staining. Results: Phylochip array analysis indicated differing microbiome composition among the diet-supplemented and the control groups, with the C. crispus group (2.5 % supplementation) showing larger separation from the control than other treatment groups. In the 2.5 % C. crispus group, the population of beneficial bacteria such as Bifidobacterium breve increased (4.9-fold, p = 0.001), and the abundance of pathogenic species such as Clostridium septicum and Streptococcus pneumonia decreased. Higher concentrations of short chain fatty acids (i.e., gut microbial metabolites), including acetic, propionic and butyric acids, were found in faecal samples of the C. crispus-fed rats. Furthermore, both C. crispus and FOS supplemented rats showed significant improvements in proximal colon histo-morphology Higher faecal moisture was noted in the 2.5 % C. crispus group, and elevated plasma immunoglobulin (IgA and IgG) levels were observed in the 0.5 % C. crispus group, as compared to the basal feed group. Conclusions: The results suggest multiple prebiotic effects, such as influencing the composition of gut microbial communities, improvement of gut health and immune modulation in rats supplemented with cultivated C. crispus.
Publication date
LanguageEnglish
AffiliationAquatic and Crop Resource Development; Measurement Science and Standards; National Research Council Canada
Peer reviewedYes
NPARC number21277050
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Record identifier602f1dee-e029-4e4c-af57-4a1d052b8a92
Record created2015-11-10
Record modified2016-05-09
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