Near-field optical probes provide subdiffraction-limited excitation areas for fluorescence correlation spectroscopy on membranes

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DOIResolve DOI: http://doi.org/10.1351/PAC-CON-08-10-10
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TypeArticle
Journal titlePure and Applied Chemistry
Volume81
Issue9
Pages16451653; # of pages: 8
Subjectbilayers; fluorescence correlation spectroscopy; near-field scanning optical microscopy; single-molecule dynamics
AbstractNear-field optical probes have been used to produce a subdiffraction-limited observation area for fluorescence correlation spectroscopy (FCS) experiments on supported membranes. The design of a bent, etched fiber probe that is compatible with biological imaging in an aqueous environment is described. This probe design is used for proof of principle experiments to measure lipid diffusion in a fluid-supported bilayer. A reduction in excitation area of approximately one order of magnitude (relative to a confocal FCS experiment) is obtained with a probe aperture diameter of 140 nm. We also demonstrate a simple approach for modeling the autocorrelation decay due to diffusion within the excitation profile at the near-field scanning optical microscopy (NSOM) probe aperture. The use of probes with smaller apertures is expected to provide an additional order of magnitude reduction in the observation area, thus enabling the study of cellular membranes with higher concentrations of fluorophores than is currently possible with diffraction-limited techniques.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NPARC number14829320
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Record identifier60d356ac-e42c-4697-ac4d-200112b4f8c0
Record created2011-02-15
Record modified2016-05-09
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