Campylobacter jejuni Biofilms Up-Regulated in the Absence of the Stringent Response Utilize a Calcofluor White-Reactive Polysaccharide: J.Bacteriol.

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TypeArticle
Journal titleJ Bacteriol.
Volume190
Issue3
Pages10971107; # of pages: 11
SubjectBacteria; bacterial; Biofilms; biosynthesis; Campylobacter; Campylobacter jejuni; Canada; capsular polysaccharide; capsular polysaccharides; crystal; DIFFERENCE; electron; GENE; Genes; IDENTIFICATION; immunology; LINKAGES; lipooligosaccharide; LIPOOLIGOSACCHARIDES; MAGNETIC; MAGNETIC-RESONANCE; Microbiology; Microscopy; MUTANT; MUTANTS; Mutation; N-linked; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; PATHOGENESIS; POLYSACCHARIDE; Polysaccharides; RESONANCE; Role; Serum; STRAIN; STRAINS; surface; Virulence
AbstractThe enteric pathogen Campylobacter jejuni is a highly prevalent yet fastidious bacterium. Biofilms and surface polysaccharides participate in stress survival, transmission, and virulence in C. jejuni; thus, the identification and characterization of novel genes involved in each process have important implications for pathogenesis. We found that C. jejuni reacts with calcofluor white (CFW), indicating the presence of surface polysaccharides harboring beta1-3 and/or beta1-4 linkages. CFW reactivity increased with extended growth, under 42 degrees C anaerobic conditions, and in a DeltaspoT mutant defective for the stringent response (SR). Conversely, two newly isolated dim mutants exhibited diminished CFW reactivity as well as growth and serum sensitivity differences from the wild type. Genetic, biochemical, and nuclear magnetic resonance analyses suggested that differences in CFW reactivity between wild-type and DeltaspoT and dim mutant strains were independent of well-characterized lipooligosaccharides, capsular polysaccharides, and N-linked polysaccharides. Targeted deletion of carB downstream of the dim13 mutation also resulted in CFW hyporeactivity, implicating a possible role for carbamoylphosphate synthase in the biosynthesis of this polysaccharide. Correlations between biofilm formation and production of the CFW-reactive polymer were demonstrated by crystal violet staining, scanning electron microscopy, and confocal microscopy, with the C. jejuni DeltaspoT mutant being the first SR mutant in any bacterial species identified as up-regulating biofilms. Together, these results provide new insight into genes and processes important for biofilm formation and polysaccharide production in C. jejuni
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMCLENNAN2008
NPARC number9371736
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Record identifier6133b39d-76c4-4899-8fe2-329895f5f7bf
Record created2009-07-10
Record modified2016-05-09
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