The mRNA transportome of the BicD/Egl transport machinery

Download
  1. (PDF, 2 MB)
  2. Get@NRC: The mRNA transportome of the BicD/Egl transport machinery (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1080/15476286.2016.1251542
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleRNA Biology
ISSN1547-6286
1555-8584
Volume14
Issue1
Pages7389
SubjectBicaudal-D; Drosophila development; egalitarian; mRNA localization; microtubule transport
AbstractmRNA (mRNA) transport focuses the expression of encoded proteins to specific regions within cells providing them with the means to assume specific functions and even identities. BicD and the mRNA binding protein Egl interact with the microtubule motor dynein to localize mRNAs in Drosophila. Because relatively few mRNA cargos were known, we isolated and identified Egl::GFP associated mRNAs. The top candidates were validated by qPCR, in situ hybridization and genetically by showing that their localization requires BicD. In young embryos these Egl target mRNAs are preferentially localized apically, between the plasma membrane and the blastoderm nuclei, but also in the pole plasm at the posterior pole. Egl targets expressed in the ovary were mostly enriched in the oocyte and some were apically localized in follicle cells. The identification of a large group of novel mRNAs associated with BicD/Egl points to several novel developmental and physiological functions of this dynein dependent localization machinery. The verified dataset also allowed us to develop a tool that predicts conserved A'-form-like stem loops that serve as localization elements in 3′UTRs.
Publication date
PublisherTaylor & Francis
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NRC numberNRC-HHT-53313
NPARC number23001893
Export citationExport as RIS
Report a correctionReport a correction
Record identifier6183f8f6-6a12-40f6-a87c-0eb1880e5607
Record created2017-05-09
Record modified2017-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)