Structural studies of the capsular polysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.

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TypeArticle
Journal titleBiochem.Cell Biol.
Volume65
Issue5
Pages414422; # of pages: 9
Subject13C; 1H; ACID; analysis; capsular polysaccharide; Chromatography,DEAE-Cellulose; Chromatography,Gas; Chromatography,Gel; Chromatography,Paper; Chromatography,Thin Layer; D-GLUCOSE; ENZYMIC; Fermentation; Glycerol; Haemophilus; Hydrofluoric Acid; Hydrolysis; immunology; isolation & purification; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methylation; MOLECULAR; Molecular Weight; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; phosphate; Pleuropneumoniae; POLYSACCHARIDE; Polysaccharides; REPEATING UNIT; RESONANCE; SEROTYPE; Serotyping; STRUCTURAL; structure; UNIT
AbstractThe capsular polysaccharide of Haemophilus pleuropneumoniae serotype 2 (ATCC 27089) is composed of D-glucose (two parts), D-galactose (one part), glycerol (one part), and phosphate (one part). Hydrolysis, dephosphorylation, methylation, enzymic studies, and 1H and 13C nuclear magnetic resonance experiments showed that the polysaccharide is a high molecular weight polymer of a tetrasaccharide repeating units, linked by monophosphate diester and having the following structure: (Formula: see text)
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberALTMAN1987E
NPARC number9369904
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Record identifier61afad49-8df2-40a4-949a-ba9bb5e4bcf9
Record created2009-07-10
Record modified2016-05-09
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