Effects of moderately oxidized dietary lipid and the role of vitamin E on the development of skeletal abnormalities in juvenile Atlantic halibut (Hippoglossus hippoglossus)

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DOIResolve DOI: http://doi.org/10.1016/j.aquaculture.2006.09.024
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TypeArticle
Journal titleAquaculture
Volume262
Issue1
Pages142155; # of pages: 14
SubjectAtlantic halibut; bone abnormalities; vitamin E; oxidized lipid; development; scoliosis; lordosis; abnormalities; acetic acid; alkaline phosphatase; antioxidants; aquaculture development; bone; defense mechanisms; dietary supplements; diets; fatty acids; feed composition; feeding; experiments; fish oils; hatcheries; hematocrit; lipids; liver; malonaldehyde; marine fish; muscles; nutritional requirements; osteoblasts; osteogenesis; oxidative stress; survival; peroxide; Hippoglossus hippoglossus
AbstractA study was conducted to characterize the effects of oxidized marine fish oil (MFO) on skeletal development in juvenile Atlantic halibut (Hippoglossus hippoglossus) and to determine the role of vitamin E on their bone health and antioxidant defense mechanisms. Juvenile halibut (4.5±0.1 g) were fed six experimental diets containing untreated (peroxide value (POV) = 0.6 meq kg-1), moderately oxidized (POV=7.5 meq kg-1) and highly oxidized (POV=15 meq kg-1) MFO either with or without a-tocopherol acetate (0 or 300 IU kg-1) supplementation for 14 weeks. No significant effects on growth, survival, hepatosomatic indices, or hematocrit were observed among the dietary treatments. Fish fed diets without vitamin E and highly oxidized dietary lipids showed increased hepatic malonaldehyde concentrations indicating a response to oxidative stress. Both muscle and liver a-tocopherol concentrations were significantly lower in fish fed diets without vitamin E supplementation. Alkaline phosphatase levels in serum and bone were increased when vitamin E was present within the diet indicating higher bone formation activity by osteoblasts. Oxidized lipids and lack of dietary vitamin E significantly increased saturated and decreased polyunsaturated hepatic fatty acids. Liver lipids of fish fed diets without vitamin E also exhibited a lower ratio of 22:6n-3 to 22:5n-3 and n-3 fatty acids. The most frequent skeletal deformity observed was scoliosis, spanning the cephalic/prehemal regions, as well as the anterior hemal region of the vertebral column, which increased the frequency according to elevated levels of oxidized dietary lipid. Lordosis was also observed, with no specific pattern along the vertebral column. The pattern and type of abnormalities observed were similar to those reported in an earlier study in halibut from a commercial hatchery. Crown Copyright © 2006 Published by Elsevier B.V. All rights reserved.
Publication date
PublisherElsevier
Copyright noticeCopyright © 2007 Elsevier B.V. All rights reserved.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number42644
1254
NPARC number3538136
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Record identifier6263cf6f-e547-4cb8-b80f-7e72072ff4c4
Record created2009-03-01
Record modified2016-05-09
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