Multiantennary group-specific polysaccharide of group B Streptococcus: Biochemistry

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TypeArticle
Journal titleBiochemistry
Volume27
Issue14
Pages53415351; # of pages: 11
Subjectanalysis; ANTIGEN; Biochemistry; biosynthesis; BOND; Canada; Carbohydrate Conformation; cell; Cell Wall; chemical; complex; COMPLEXES; COMPONENT; Form; group B Streptococcus; GROUP-B; Hydrolysis; linear; Linker; Magnetic Resonance Spectroscopy; MOLECULE; oligosaccharide; Oligosaccharides; POLYSACCHARIDE; Polysaccharides; RESIDUES; SEQUENCE; Streptococcus; Streptococcus agalactiae; structure; sugar; SUGARS; Support,U.S.Gov't,P.H.S.; UNIT
AbstractThe group-specific antigen of group B Streptococcus is composed of four different oligosaccharide units of Mw 766 (III), 1277 (II), 1462 (IV), and 1788 (I). The major constituent sugars of the oligosaccharides are alpha-L-rhamnopyranose, alpha-D-galactopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranosyl, and D-glucitol except that III does not contain alpha-D-galactopyranosyl or 2-acetamido-2-deoxy-beta-D-glucopyranosyl residues and IV contains no D-glucitol but has one additional beta-L-rhamnopyranosyl residue. The structures of II and III have been previously elucidated [Michon, F., Katzenellenbogen, E., Kasper, D. L., & Jennings, H. J. (1987) Biochemistry 26, 476-486]. In the group B antigen all the oligosaccharides are linked by one type of phosphodiester bond from O6 of the D-glucitol residue of one oligosaccharide to O6 of the alpha-D-galactopyranosyl residue of the next to form a complex and highly branched multiantennary structure. However, despite the heterogeneous nature of its component oligosaccharides, some order has been identified in the biosynthesis of the group B antigen from chemical and enzymatic sequence studies. Because III lacks an alpha-D-galactopyranosyl residue but has a D-glucitol residue, it is situated at the reducing terminus of all the branches of the group B antigen where it is always adjacent to a II moiety. Conversely, IV has an alpha-D-galactopyranosyl residue but has no D-glucitol and is therefore located at the reducing terminus of the group B antigen where it probably functions as a linker molecule between the group B polysaccharide and the cell wall peptidoglycan of the group B streptococcal organisms. Oligosaccharide I contains two alpha-D-galactopyranosyl residues and one D-glucitol residue and thus constitutes the branch point in the group B antigen, whereas II contains one of each of the above residues and therefore is situated in linear interchain positions. The group B antigen is highly branched and probably has a unique multiantennary structure
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMICHON1988A
NPARC number9365296
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Record identifier628e93e4-ae69-4a2f-9746-38c791b956a3
Record created2009-07-10
Record modified2016-06-01
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