The structural diversity of lipopolysaccharide expressed by non-typeable Haemophilus influenzae strains 1158 and 1159

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2012.04.023
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume357
Pages98110
SubjectHaemophilus influenzae, Lipopolysaccharide, Globotetraose, Sialyllactose, Phosphocholine
AbstractA heterogeneous population of glycoforms expressed by NTHi strains 1158 and 1159 has been elucidated using NMR spectroscopy and capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) materials, as well as HPLC-ESI-MSn on dephosphorylated and methylated OS samples. The most abundant glycoform contained a disaccharide chain: PCho→7)-D-α-D-Hepp-(1→6)-β-D-Glcp linked to HepI from the common structural element of H. influenzae LPS: L-α-D-HepIIIp-(1→2)-[PEtn→6]-L-α-D-HepIIp-(1→3)-L-α-D-HepIp-(1→5)-[PPEtn→4]-α-Kdop-(2→6)-lipid A. Phosphocholine (PCho) was found at two positions in the LPS glycoforms; PCho substituted the 6-position of β-D-Glcp attached to HepIII and was also located at a novel position linked to D-α-D-Hepp; this latter position was determined by structural analysis of LPS from a 1158lpsA mutant strain. Additionally, HPLC-ESI-MSn experiments indicated glycoforms that have chain elongation from HepII, this was found only in glycoforms, which lack the additional heptose in the outer core region. Structural details of these glycoforms were confirmed by analyses of LPS from a 1158losB2 mutant strain; the losB2 gene is required for addition of the d,d-Hep to the outer core region in strain 1158.
Publication date
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
IdentifierS0008621512002017
NPARC number21268688
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Record identifier649c7b7c-5069-4f8c-8ae7-c8063b696d14
Record created2013-11-07
Record modified2016-05-09
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