Different iron-chelating properties of pyochelin diastereoisomers revealed by LC/MS

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DOIResolve DOI: http://doi.org/10.1007/s00216-006-0443-0
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume385
Issue3
Pages606611; # of pages: 6
Subjectpyochelin; pseudomonas siderophore; iron complex; electrospray ionization; liquid chromatography/mass spectrometry
AbstractPyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. It is isolated from bacterial media as a mixture of two epimers, which readily equilibrate in most solvents. Experiments based on high-performance liquid chromatography/electrospray ionization mass spectrometry are reported here, allowing the investigation of the different Fe(III)-chelating properties of pyochelin diastereomers in solution without the need for labourious isolation. It is demonstrated in this study that only one of the two pyochelin diastereomers is able to chelate Fe(III); no Fe(III) complexes of the other diastereomer could be detected. The Fe(III)-pyochelin complex exhibited a 1:1 metal-to-siderophore ratio and no evidence for other stoichiometries was found.
Publication date
PublisherSpringer-Verlag
Copyright noticeCopyright © 2007, Blackwell Publishing Ltd.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NRC number55867
1527
NPARC number3538405
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Record identifier6568105e-258d-4fcf-bc00-029e7464c65f
Record created2009-03-01
Record modified2016-05-09
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