Highly conserved Neisseria meningitidis inner-core lipopolysaccharide epitope confers protection against experimental meningococcal bacteremia: J.Infect.Dis.

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TypeArticle
Journal titleJ.Infect.Dis.
Volume187
Issue8
Pages12231234; # of pages: 12
SubjectAntibodies; antibody; assay; Bacteremia; DISEASE; epitope; immunology; In Vitro; Infant; LIPOPOLYSACCHARIDE; LPS; MOLECULAR; MONOCLONAL-ANTIBODIES; MONOCLONAL-ANTIBODY; Neisseria; Neisseria meningitidis; Prevalence; PROTECTION; Rats; STRAIN; STRAINS; TARGET
AbstractInner-core lipopolysaccharide (LPS) from Neisseria meningitidis is under investigation as a vaccine for prevention of meningococcal disease caused by N. meningitidis serogroup B (NmB). We investigated the functional activity of murine monoclonal antibody (MAb) B5 that recognizes a highly conserved (galE) LPS epitope. Three patterns of MAb reactivity were observed in N. meningitidis by Western blot, depending on the relative prevalence of sialylated, nonsialylated, and/or truncated LPS glycoforms. Three representative N. meningitidis strains (8047, M986, and 2996) were investigated with MAb B5 in functional assays in vitro and in vivo. MAb B5 completely protected infant rats against bacteremia caused by 8047, partially protected against 2996, and had no protective activity against M986. Thus, an inner-core LPS epitope can be a target for protective immunity, but the affinity of MAb B5 may only be sufficient to mediate protection against NmB strains possessing at least some truncated glycoforms
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberPLESTED2003
NPARC number9379812
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Record identifier6613278a-eb23-4935-a273-1c1fa79d3da9
Record created2009-07-10
Record modified2016-05-09
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